Effects of Culture System and Substrate Composition on Micropropagated Plantlets of Two Varieties of Stevia rebaudiana Bert

Background: Secondary metabolites in stevia leaves offer important therapeutic benefits. The germination problems of seeds and the high heterogeneity resulting populations make micropropagation preferred tool for obtaining a large number plants short time. Until now, studies have focused on optimizing action growth regulators improvement micropropagated plantlets. In this project, we study alternative mineral nutrients substrate two culture systems plantlets to obtain proper amounts enhanced Methods: Criolla Morita varieties; MS, MSM, G substrates, temporary immersion (TIS) agar-medium were used. Biometric variables damage cell membrane tested. Results: More productivity growing MS solid medium TIS. presented higher substrates explants MSM highest productivity. was generally better than No found. Conclusions: efficiency varieties has been without negative effects their physiological condition. Consequently, any used, including bioreactors, can be industrial application scale.